SIMPLIFIED EXTRACTION METHODS FOR THE RAPID DETERMINATION OF SPECIES CONTENT OF ADIPOSE TISSUE BASED ON THE DETECTION OF TNI IN IMMUNOASSAYS

摘要

A method for extracting protein from a fat-containing sample is described comprising the steps of separating solidified fat and solid residues from a centrifuged mixture of protein, fat, other solid materials and aqueous solution of phosphate buffered saline to form an aqueous phase containing the protein. The aqueous phase is then filtered through a filter to separate a clear protein extract from the mixture.