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EXPRESSION CONSTRUCT FOR SENSING CELL DENSITY AND SUBSTRATE AVAILABILITY AND ITS USE IN CONVERSION OF HYDROXYCINNAMIC ACIDS
EXPRESSION CONSTRUCT FOR SENSING CELL DENSITY AND SUBSTRATE AVAILABILITY AND ITS USE IN CONVERSION OF HYDROXYCINNAMIC ACIDS
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机译:感知细胞密度和基质利用率的表达构建及其在羟基肉桂酸转化中的应用
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摘要
An Expression system; isolated nucleic acid molecule or host cell comprising: (i) A first gene encoding for a first enzyme linked to a first promoter, wherein the first promoter is a time delay promoter; (ii) A second gene encoding for a second enzyme capable of using the product generated by the first enzyme as a substrate, wherein the second gene is operably linked to a second promoter, wherein the second promoter is inducible by the product generated by the first enzyme; (iii) Optionally, a third gene encoding a transcription factor that represses expression of the second gene in the absence of the product generated by the first enzyme, wherein the third gene is operably linked to a third promoter that regulates expression of the third gene; and its use in producing a product such as hydroxybenzaldehyde.
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