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Expression vector for expression of eukaryotic secretion leader sequence fused with a target polypeptide and methods of use thereof
Expression vector for expression of eukaryotic secretion leader sequence fused with a target polypeptide and methods of use thereof
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机译:用于表达与靶多肽融合的真核分泌前导序列的表达载体及其使用方法
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摘要
An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.
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