METHODS AND KITS FOR DETECTION OF O-GLCNAC AND N-GLCNAC MODIFICATION OF PEPTIDES AND PROTEINS

摘要

Methods and kits for detecting the presence of GlcNAc modification of a peptide or protein. The steps of detection may include combining the peptide or protein with PAP³⁵S and a sulfotransferase to produce a reaction product including ³⁵S labeled GlcNAc modified protein or peptide, removing background labeling with a glycosidase, separating the ³⁵S labeled GlcNAc modified protein or peptide from PAP³⁵S, free S-35 sulfate and labeled oligosaccharides if present, and detecting isotopic emissions from the ³⁵S labeled GlcNAc modified peptide or protein. The detection may further include, prior to detection, the step of combining the peptide or protein with a GlcNAc transferase and UDP-GlcNAc under appropriate conditions for the GlcNAc transferase to transfer a GlcNAc to the peptide or protein.