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METHOD FOR NON-FREEZE LOW-TEMPERATURE PRESERVATION OF MAMMALIAN EMBRYO OR FERTILIZED EGG
METHOD FOR NON-FREEZE LOW-TEMPERATURE PRESERVATION OF MAMMALIAN EMBRYO OR FERTILIZED EGG
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机译:哺乳动物胚胎或受精卵的非冷冻低温保存方法
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摘要
Disclosed is a novel means which enables satisfactory preservation of embryos and fertilized eggs in the non-frozen state for a longer period than conventional means, which novel means also achieves high hatching ability and a high conception rate of the embryos after the preservation. The method for preserving a mammalian embryo(s) or fertilized egg(s) of the present invention comprises immersing a mammalian embryo(s) or fertilized egg(s) in a medium containing 20 to 80% (v/v) serum and 10 to 100 mM Good's buffer, and storing the embryo(s) or fertilized egg(s) at non-freezing low temperature. The preservative solution for a mammalian embryo(s) or fertilized egg(s) of the present invention essentially consists of a medium containing 20 to 80% (v/v) serum and 10 to 100 mM Good's buffer. The Good's buffer is preferably HEPES.
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机译:公开了一种新颖的方法,与常规方法相比,该方法能够令人满意地以非冷冻状态长时间保存胚胎和受精卵,该新颖方法还实现了高孵化能力和保存后胚胎的高受孕率。本发明的保存哺乳动物胚胎或受精卵的方法包括将哺乳动物胚胎或受精卵浸入含有20至80%(v / v)血清和10的血清的培养基中。到100 mM Good's缓冲液中,并在非冰冻的低温下保存胚胎或受精卵。本发明的用于哺乳动物胚胎或受精卵的防腐剂溶液基本上由含有20至80%(v / v)血清和10至100mM Good's缓冲液的培养基组成。货物的缓冲器最好是HEPES。
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