METHOD FOR NON-FREEZE LOW-TEMPERATURE PRESERVATION OF MAMMALIAN EMBRYO OR FERTILIZED EGG

摘要

Disclosed is a novel means which enables the satisfactory preservation of a mammalian embryo and a mammalian fertilized egg in a non-frozen state for a longer period compared with conventional means and also enables the hatchability and the conception rate of the embryo after the preservation to be maintained at higher levels. A method for preserving a mammalian embryo or fertilized egg according to the present invention comprises: immersing the mammalian embryo or fertilized egg in a culture medium containing 20 to 80% (V/V) of serum and 10 to 100 mM of a Good's buffer; and then preserving the embryo or fertilized egg under non-freeze low-temperature conditions. A preservation solution for a mammalian embryo or fertilized egg according to the present invention is essentially composed of a culture medium containing 20 to 80% (V/V) of serum and 10 to 100 mM of a Good's buffer. The Good's buffer is preferably HEPES.