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Closed system separation of adherent bone marrow stem cells for regenerative medicine applications
Closed system separation of adherent bone marrow stem cells for regenerative medicine applications
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机译:封闭系统分离粘附的骨髓干细胞,用于再生医学应用
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摘要
A method for isolating and processing bone marrow derived stem cells, including the steps of: (a) collecting a biological sample containing adherent bone marrow stem cells in a receptacle with interior walls coated with a cell-adherent substrate; (b) incubating the bone marrow cells on the adherent substrate so that a layer of adherent bone marrow stem cells adheres to the substrate; (c) washing any non-adherent cells from the substrate; and (d) collecting the bone marrow stem cell layer. Isolation kits and use of bone marrow cells harvested for cell therapies are also described.
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