Microscope, method and computer program for quantitative Phase Imaging by means of digital holographic Microscopy and Kit for Adjusting an optical microscope

摘要

Microscope, method and computer program for Imaging Volume Phase by means of digital holographic Microscopy, and a kit to adapt microscopiou00f3pticoel Microscope comprises a coherent Light Source (1) and a Beam Splitter (3) to generate an object Beam ( ) for illuminating a SampleAnd a reference Beam (LR); an Optical System with an optical path which is a Main ytelecu00e9ntrico afocal System, a reference Optical Path; and a means of Recording (12) with a Hologram that shows in the planoimagen System comprises u00f3ptico.el method Recording a Hologram On the image plane of a telecentric system and u00f3pticoafocal.The computer program is adapted to implement part of the stages of the mu00e9todo.el kit includes a means for varying the inclination angle of a reference Beam.