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Quantitative phase-contrast imaging with compact digital holographic microscope employing Lloyd's mirror

机译:使用劳氏镜的紧凑型数字全息显微镜进行定量相衬成像

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Digital holographic microscopy (DHM) is one of the most effective techniques used for quantitative phase imaging of cells. Here we present a compact, easy to implement, portable, and very stable DHM setup employing a self-referencing Lloyd's mirror configuration. The microscope is constructed using a diode laser source and a CMOS sensor, making it cost effective. The reconstruction of recorded holograms yields the amplitude and phase information of the object. The temporal stability of the presented technique was found to be around 0.9 nm without any vibration compensation, which makes it ideal for studying cell profile changes. This aspect of the technique is demonstrated by studying membrane fluctuations of red blood cells.
机译:数字全息显微镜(DHM)是用于细胞定量相成像的最有效技术之一。在这里,我们介绍了一种紧凑,易于实现,便携式且非常稳定的DHM设置,它采用了自参考劳埃德(Lloyd)镜结构。该显微镜使用二极管激光源和CMOS传感器构成,因此具有成本效益。记录的全息图的重建产生物体的振幅和相位信息。发现所提出的技术的时间稳定性为0.9nm左右,没有任何振动补偿,这使其成为研究细胞分布变化的理想选择。通过研究红细胞的膜波动来证明该技术的这一方面。

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