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Method. In the detection of targets, the historical responsibility is from the point of view.

机译:方法。在检测目标中,历史责任是从角度来看的。

摘要

The invention provides a method for directly detecting a biological fragment of a nucleic acid fragment from a target. Or by in situ hybridization. The method includes several steps, and it should be based on the kappa alpha tau.A biological preparation sample was deposited on a slide microscope. The sample on the slide is stable with an unstable microscope, including methanol acetic acid or stabilized in one. Reason: if it is from 991~80: 20 formalin acetic acid, or from 99 to 1~80: 20.Stable nucleic acid samples are exposed to a specific segment of the composite sensor under the condition of target nucleic acid. It is hybridized with lambda. The sensor is from the sample. The epsilon of the sample is Evans blue.The sensor is a sign of T in the presence of the complex of_beta p_delta_pi__u_under the optical microscope. The nucleic acid of your target fragment. This method can achieve enough hybridization in different buffer solutions, which reveal.The method of the invention is useful for detecting biological agents of pathogenic bacteria. The concrete complexes reveal that the sensor is used to detect the pathogen Mu PI alpha_alpha (Babesia). The method is useful in detecting biological agents, including serum, from a wide range of nucleic acids.
机译:本发明提供了一种直接检测来自靶标的核酸片段的生物学片段的方法。或通过原位杂交。该方法包括几个步骤,应该基于κατ。将生物制剂样品沉积在载玻片显微镜上。载玻片上的样品在不稳定的显微镜下(包括甲醇,乙酸或稳定在其中)是稳定的。原因:是从991〜80:20的福尔马林乙酸,还是从99到1〜80:20。在目标核酸的条件下,稳定的核酸样品会暴露于复合传感器的特定片段。它与λ杂交。传感器来自样品。样品的ε是埃文斯蓝。在光学显微镜下,存在β-p_delta_pi__u_的复合物时,传感器是T的信号。您的目标片段的核酸。该方法可以在不同的缓冲溶液中实现足够的杂交,这表明。本发明的方法可用于检测病原细菌的生物因子。具体的配合物表明该传感器用于检测病原菌Mu PI alpha_alpha(巴贝西亚)。该方法可用于检测多种核酸中的生物试剂,包括血清。

著录项

  • 公开/公告号CY1110164T1

    专利类型

  • 公开/公告日2015-01-14

    原文格式PDF

  • 申请/专利权人 IGENEX INC.;

    申请/专利号CY201011000590T

  • 发明设计人 HARRIS NICK S;SHAH JYOTSNA S;

    申请日2010-06-28

  • 分类号C12Q1/68;C07H21/02;C07H21/04;C12N15/00;C12Q1/6841;C12Q1/6888;C12Q1/6893;

  • 国家 CY

  • 入库时间 2022-08-21 15:15:49

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