A NOVEL PROCESS OF SPORULATION, ACTIVATION AND GERMINATION IN THERMOPHILIC BACTERIA FOR RAPID DETECTION OF ANTIBIOTIC RESIDUES IN MILK

摘要

This invention relates to a novel analytical process of detection of antibiotic residues in milk system involving sporulation upto 80-90% in thermophillic strains B. stearothermophilus seeded in selective sporulation medium containing Beef extract 0.15-0.30% preferably 0.3%; peptone 0.25-0.5% preferably 0.5%; tryptone .09-0.17% preferably 0.17%; sodium chloride 0.025-0.5% preferably 0.05%; agar 1.5+0.2% preferably 1.5%; brombcresol purple .004-.008% preferably .006% as color indicator, followed by activation of dormant spores of B. stearothermophilus in BPTS medium at 55±5°C for 12+3 hours preferably at 55°C for 14 hours incubation, wherein the activated spores are forced for germination and out growth using 0.5+0.05mg D-dextrose preferably 0.5 mg; 5.0+0.5mg skimmed milk powder preferably 5.0 mg; 2.0±0.2mg whey powder preferably 2.0 mg at incubation temp. 64±2°C preferably at 64°C as germinant, wherein change of colour of the medium from purple to yellow within 2-3 hours is taken as criteria of dormant spores into vegetative cells and finally mixing germinant mixture 55+5.0 mg preferably 60 mg in milk and heated to 95 to 105°C preferably 100°C for 2-3 minutes and adding .01-.02 ml preferably .015 ml of milk in BPTS medium and incubating at 64±2°C preferably 64°C for 2.30-3 hours and observing the change of color of the medium.