Rapid Detection of β-lactam Antibiotic Residue in Milk Using Biolayer Interferometry

摘要

[Objective]This study aimed to establish a high-sensitivity method for rapid detection of β-lactam antibiotic residue in milk. [Method]Based on biolayer interferometry technology,ampicillin-BSA conjugate was fixed on the bottom of APS fiber optic biosensor probe through hydrophobic interaction and bound to40 nm colloidal gold-labeled β-lactam antibiotic receptor,to detect β-lactam antibiotics in milk. [Result]The sensitivity of colloidal gold-labeled BLI method was twice as high as that of immunochromatographic test strip in detection of β-lactam antibiotic residue in milk. Colloidal gold-labeled BLI method exhibited good specificity and had no cross-reaction with 1 000 ng/ml aflatoxin M1,gentamicin,kanamycin,streptomycin,tylosin,chloromycetin and melamine. [Conclusion] The colloidal gold-labeled BLI method is not suitable for quantitative detection in actual production due to its small quantitative range in detection of β-lactam antibiotics,but it is a simple and rapid qualitative detection method that can be used in rapid detection of β-lactam antibiotic residue in milk.