A CYCLIC LIPOPOLYPEPTIDE OF BACILLUS SUBTILIS SSP SUBTILIS (VCRC B4721) WITH POTENTIAL TO KILL MOSQUITO STAGES

摘要

This invention relates to a product from a bacterium, Bacillus subtilis ssp. subtilis (VCRC B471) isolated from soil samples, useful for controlling mosquitoes by killing their immature stages and adults. The product is obtained based on subculturing of the bacterium on nutrient yeast salt medium agar (NYSMA), inoculating slant (NYSM) culture thus obtained into NYSM broth and incubating for obtaining seed, inoculating NYSM broth with seed thus obtained, followed by centrifuging the culture for separation of bacterial cells and cell free culture medium, separation of the bacterial metabolite from the cell free culture medium by acid precipitation and its lyophilisation. The product thus obtained and/ or made into suitable formulation has the potential to kill mosquito larvae, pupae and adult. The product incorporated into vaporizing mat or liquid vaporizer or aerosol has the potential to kill adult mosquitoes.