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REAL TIME VIRAL DETECTION METHOD AND KIT FOR INFECTIOUS HYPODERMALAND HAEMATOPOIETIC NECROSIS VIRUS
REAL TIME VIRAL DETECTION METHOD AND KIT FOR INFECTIOUS HYPODERMALAND HAEMATOPOIETIC NECROSIS VIRUS
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机译:感染性低聚体造血坏死病毒的实时病毒检测方法和试剂盒
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摘要
A method for quantitatively detecting infectious hypodermal and haematopoietic necrosis virus (IHHNV) in a biological sample, comprising the steps of obtaining a primer pair having nucleotide sequences set forth in SEQ ID NO: 1 and SEQ ID NO: 2, and a fluorescent probe having a nucleotide sequence set forth in SEQ ID NO: 3; amplifying a non-structural protein coding region of the virus in the biological sample by a real-time polymerase chain reaction (PCR) using the primer pair and the fluorescent probe; and determining the presence and quantity of the virus in the biological sample with reference to a positive control plasmid.
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机译:一种用于定量检测生物样品中的感染性皮下和造血坏死病毒(IHHNV)的方法,该方法包括以下步骤:获得具有SEQ ID NO:1和SEQ ID NO:2所示核苷酸序列的引物对,以及具有SEQ ID NO:3所示的核苷酸序列;使用引物对和荧光探针通过实时聚合酶链反应(PCR)扩增生物样品中病毒的非结构蛋白编码区;并参照阳性对照质粒确定生物样品中病毒的存在和数量。
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