Method for producing vaccines using recombinant DNA technology immunological veterinary medicinal product for domestic pigeons

摘要

the subject of the invention is a way to produce immunological veterinary medicinal product for domestic pigeons (columbia livia) vaccine using recombinant dna technology przeciwkabout cirkowirusowej disease wywolywanej by cirkowirus doves pn. picv (ang.pigeon circo virus) which has, gets married with the organ material from infected pigeons petthen isolates the material's genetic by isolation calkowitego dna viralduplicates the structural protein gene kapsydu (orf c1) virus picv about the greatness of 822 (or its shortened form) to clone protein orf c1 or its abbreviated form gene orf c1 to the vector pgem or other systemu00f3in ekspresyjnych nucleotide and amino acid sequences and determines summary form gene orf c1wklonowuje's short form gene orf c1 to the genome vector pqe - 31 - or vector pet 32a (or other systems ekspresyjnych) and obtains the expressions of recombinant protein to structural of e.coli or insect cells or eukaryotic. the vaccine may contain additionally selected antigens or combinations of antigens from pathogens isolated from pigeons as salmonella spp., mycoplasma spp.paramyksowirus, bird - 1 (pmv - 1)herpeswirus doves - 1 (pihv) and authorised for use in clinical veterinary nosniki or rozcienczalniki and adjuvants and immune substances wzmagajace answer.