Method for producing PHAs by a consortium of methane bacteria colonizing the sedimentary rocks

摘要

the subject of the invention is a manufacturing polihydroksyalkanolanu00f3w (pha) with methane by bacterial consortium zasiedlaju0105ce rocks przywu0119glowe.the way is that the rock przywu0119glowu0105 first is chopped to about? 2 mm and subjected preinkubacji in temperature from 10 to 30 oc with a moisture content of 25 to 200% of the total volume of water in an atmosphere containing 1 - 50% ch4 v / v, then the rock inokuluje medium nms, containing from 0.1 to 1 g / dm3 n - no3 and incubated at a temperature of 20 to 30 oc,in an atmosphere containing 1 - 50% ch4 v / v stirring constantly until the stationary phase culture growth. in turn, cultured bacteria is separated from the substrate and isolates the pha in two stages.in the first stage of the cells of the bacteria are removed by extraction with methanol fats and dyes for 1.5 hours at 120 degrees, and subsequently included in the mass of bacterial phb is extracted with boiling chloroform by 1.5 hours at 70u00b0c, then after odfiltrowani in pollution phb precipitates with methanol and then take nobody nowhere and drought.