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Genetically coded FRET-based biosensor of MMP-9 activity and its application
Genetically coded FRET-based biosensor of MMP-9 activity and its application
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机译:基于遗传密码的基于FRET的MMP-9活性生物传感器及其应用
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摘要
the invention concerns genetically encoded fret phenomenon, using biosensora to monitor activity metaloproteazy extracellular matrix 9 (mpp) 9. mpp - 9 is involved in extracellular endopeptydazu0105 both physiological and pathological processes.anchored in the plasma membranes, genetically encoded, using fret phenomenon, biosensor allow testing activity proteolitycznej mpp - 9 with high resolution temporarily - spatial directly in place of the mpp - 9 on the cell.the functionality biosensora, both in vitro and in vivo in living cells was demonstrated by analysis of ratiometrycznej cuts biosensora by purified auto aktywuju0105cu0105 mutated mpp - 9.
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