METHOD TO IMPROVE PLANTS ENDURANCE TO NITROGEN STRESS

摘要

The present invention provides the method to improve the plants’ endurance to nitrogen stress. The method includes the inhibition of the synthesis of ethylene in the plant by stable transformation of the plant using heterologous polynucleotide that reduces the expression of ACC-synthase with the expression of the specified heterologous polynucleotide and growing of the specified plant under the conditions of limited nitrogen, whereby the specified heterologous polynucleotide is expressed and the specified plant reveals the plants’ improved endurance to nitrogen stress in comparison with the observational plant. The specified heterologous polynucleotide contains nucleic acid which encodes the transcript that is able to form a double-stranded RNA and mediate RNA interference of the nucleic acid of ACC-synthase. The specified nucleic acid contains first nucleotide sequence that contains at least 21 contiguous nucleotides of the nucleic acid of ACC-synthase and second nucleotide sequence containing complementary sequence of the specified first nucleotide sequence, wherein the first and second nucleotides are separated by nitron.