A METHOD FOR RECEIVING ASEPTIC CULTURE RIBES NIGRUM L. IN CONDITIONS IN VITRO

摘要

A method for receiving aseptic culture Ribes nigrum L. in conditions in vitro, which includes aseptic culture explants annual shoots of currants apical and lateral buds taken from the crown of the bush, soap solution, 0.1% HgCl2 solution and sterile dH2O. Shoots 1,5-3,0 cm long stages in sterilized solution of detergent 15 min., Exercise, money in running water 20 min., Transfer to a sterile dH2O, then - a solution C2H5OH - 70% (30 seconds)., HgCl2 - 0.1% (15 min.) money in sterile dH2O three times for 10 min., while explants separated into fragments of stems 1.0-1.5 cm long, 1 bud.