Methods and compositions for the production of recombinant protein in mammalian cells expressing HBx

摘要

A method of producing a heterologous protein in cultured mammalian host cells, comprising culturing mammalian host cells selected from the group consisting of BHK21 cells, wherein said mammalian host cells contain a nucleic acid encoding HBx, a nucleic acid providing exogenous XBP1s and a nucleic acid encoding the heterologous protein, under conditions such that HBx and the heterologous protein are expressed by the mammalian host cells.