Procedure for identifying modulators of catechol O-methyltransferase

摘要

A method for identifying a modulator of the activity of the enzyme catechol O-methyltransferase (COMT) comprising the steps of: a) providing 4-nitrocatechol covalently linked with Alexa Fluor 488 having formula I, b) contacting the molecule of step a) with a methyltransferase O (COMT) enzyme catechol, Sadenosilmetionina (SAM) and a compound c candidate) measuring the fluorescence reading of the mixture of step b), wherein fluorescence reading altered in the presence the candidate compound compared to a target is indicative of a modulator of catechol O-methyltransferase (COMT). ** ** Formula