METHOD OF IDENTIFYING CATECHOL O-METHYLTRANSFERASE MODULATORS

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to medicine and describes method of identifying modulators of enzyme catechol-O-methyltransferase (COMT) activity, including a) obtaining 4-nitrocatechol, covalently bound with Alexa Fluor® 488, b) bringing molecule from stage a) in contact with enzyme catechol-O-methyltransferase (COMT), S-adenosylmethionine (SAM) and candidate compound and c) measurement of indices of fluorescence of mixture from stage b), in which changed indices of fluorescence in presence of candidate compound in comparison with the control serve as sign of presence of modulator of enzyme catechol-O-methyltransferase (COMT). Also described is method of identifying substrate of enzyme catechol-O-methyltransferase (COMT).;EFFECT: invention can be used for identification of compounds, inhibiting enzyme COMT, to determine COMT activity in samples of animal tissues.;10 cl, 3 ex, 7 dwg