SELF-CALIBRATING GRADIENT DILUTION IN A CONSTITUENT ASSAY AND GRADIENT DILUTION APPARATUS PERFORMED IN A THIN FILM SAMPLE

摘要

A method and apparatus for measuringan-tibody titers in a thin film sample in an automated systemwhich does not require multiple dilutions. The systempro-vides a simple method for creating an in-situ dilutionwithin a sample analysis chamber without the use of anyprecision fluid-handling components, and further, to usethe same principles to provide a wide range of sampledi-lutions within the chamber so as to obviate the need foradditional dilution steps when dealing with samplespossi-bly containing wide ranges of analyte concentrations.