ENGINEERED MICROORGANISMS WITH IMPROVED GROWTH PROPERTIES

摘要

The present application relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial pyruvate-derived metabolites, e.g., isobutanol. In some embodiments, the recombinant yeast microorganisms may be is engineered to comprise reduced PDC activity. In additional embodiments, the recombinant yeast microorganisms may be engineered to (a): comprise at least one first modification which results in improved growth in the absence of C2-supplementation as compared to the corresponding recombinant yeast microorganism without said first modification; and/or (b) comprise at least one second modification which results in improved tolerance to glucose as compared to the corresponding recombinant yeast microorganism without said second modification.