PREPARATION METHOD FOR ISOLATED, PURIFIED BEE VENOM HAVING ALLERGIC COMPONENTS ISOLATED

摘要

The present invention relates to a preparation method for isolated, purified bee venom having allergic components isolated, capable of preparing the isolated, purified bee venom fundamentally blocking allergy induction while maintaining a pharmacological effect by reducing or removing allergy-inducing components in bee venom, as an ultrafiltration method. The present invention comprises: a first step for arranging purified bee venom powder; a second step for preparing a bee venom solution by mixing the purified bee venom powder with distilled water; a third step for removing the allergic components included in the bee venom solution by filtering the bee venom solution through an ultrafiltration membrane having a cut-off size of 10kDa (kilo-Dalton); and a fourth step for harvesting the isolated, purified bee venom having the allergic components removed, wherein, in the second step, the distilled water being mixed with the purified bee venom powder corresponds to 1000ml to 1500ml per 1g of the purified bee venom powder, in the third step, the allergic components removed through the ultrafiltration membrane are A2 (phospholipase A2) and hyaluronidase, and, in the fourth step, the harvested isolated, purified bee venom comprises 4% by weight or more of apamin and 50% by weight or more of melittin.