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RNA POLYMERASE, METHODS OF PURIFICATION AND METHODS OF USE
RNA POLYMERASE, METHODS OF PURIFICATION AND METHODS OF USE
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机译:RNA聚合酶,纯化方法和使用方法
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摘要
Cyanophage Syn5 RNA polymerase (RNAP) and methods of purifying it are provided. Cyanophage Syn5 RNAP having one or more promoter mutations are provided. Methods of expressing cyanophage Syn5 RNAP are provided. Novel promoter sequences and in vitro transcription systems utilizing Syn5 RNAP are provided. Methods of making high range ssRNA ladders are also provided. Methods of incorporating 2'-modified dNTPs using Syn5 RNA polymerase are provided.
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