SMALL RNA, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF IN PHARMACEUTICALS FOR SPECIFICALLY UP-REGULATING GENE TRANSCRIPTIONAL ACTIVITY

摘要

Disclosed in the present invention are a method for specifically up-regulating gene expression through a target core promoter using small RNAs (including microRNAs and small interfering RNAs), and a series of regulation target genes. The present invention sets forth that when a TATA box sequence is contained in the promoter, a target site is in a range defined by upstream extension of 20 bases from and downstream extension of 20 bases from the TATA box sequence serving as a center; when no TATA box sequence is contained in the promoter, the target site is an upstream sequence of 1-50 with respect to the genetic transcription starting site. The microRNAs hsa-let-7i, hsa-miR-138, hsa-miR-92a, hsa-let-7c and hsa-miR-181d specifically up-regulate the expression of interleukin-2, insulin, thyrocalcitonin, histone and c-myc gene, respectively. Besides, in regard of randomly selected 19 gene promoters, the artificially-synthesized small interfering RNAs (siRNAs) can enhance the transcriptional activities of 78.9% of the genes. An objective of the present invention is to provide a method for specifically up-regulating gene expression using micro-RNAs, and the present invention possesses a high application value and a broad application prospect in the fields of biotechnology and biomedicine.