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HUMAN KNEE-JOINT CARTILAGE CELL IN-VITRO AMPLIFICATION METHOD FOR CLINIC TREATMENT
HUMAN KNEE-JOINT CARTILAGE CELL IN-VITRO AMPLIFICATION METHOD FOR CLINIC TREATMENT
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机译:人膝关节软骨细胞的体外扩增方法
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摘要
Provided is a human knee-joint cartilage cell in-vitro amplification method for clinic treatment. The method comprises the following steps: separating and extracting in-vitro cartilage of a patient, and obtaining cartilage cells; inoculating the cartilage cells to a culture medium to perform primary cell culture; and performing cell subculture when the confluence degree of the cartilage cells in the primary cell culture is 70-80 percent, the culture medium for performing primary cell culture and cell subculture comprising patient in-vitro serum with the volume fraction of 5-20 percent, 20-40ng/ml of FGF and 20-40ng/ml of PDGF.
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机译:提供了一种用于临床治疗的人膝关节软骨细胞体外扩增方法。该方法包括以下步骤:分离并提取患者的体外软骨,并获得软骨细胞。将软骨细胞接种到培养基中以进行原代细胞培养;在原代细胞培养中软骨细胞的融合度为70-80%时进行细胞亚培养,所述原代细胞培养和细胞亚培养的培养基中含有体积分数为5%至20%的患者体外血清, 20-40ng / ml FGF和20-40ng / ml PDGF。
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