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METHOD FOR AMPLIFYING CD8+T CELLS AND CELL SUBPOPULATIONS THEREOF IN-VITRO
METHOD FOR AMPLIFYING CD8+T CELLS AND CELL SUBPOPULATIONS THEREOF IN-VITRO
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机译:体外扩增CD8 + T细胞及其细胞亚群的方法
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摘要
Disclosed is a method for rapidly amplifying CD8+ T cells and functional cell subpopulations thereof in vitro. A TLR1/2 agonist, a TLR2/6 agonist and a TLR5 agonist or a combination of above agonists is added to a conventional culture system for in-vitro amplification of CD8+ T cells. Recombinant cytokines IL-2, IL-7 and IL-15 as well as magnetic beads coated with an anti-human CD3 antibody and an anti-human CD28 antibody can be further added to the culture system for continuous co-stimulation.
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