TUMOR-ASSOCIATED MACROPHAGES AND METHODS AND COMPOSITIONS FOR TARGETING CANCER THERAPY AND IDENTIFYING POTENTIAL RESPONDERS

摘要

Disclosed are methods for stratifying a subject suffering from a solid tumor according to a percentage of cytotoxic CD8+ tumor associated T cells and employing such information in identifying whether the patient is a suitable candidate for the treatment with immune checkpoint inhibitors such as PD-1 or PD-Ll. The method comprises (a) assessing the percentage of cytotoxic phenotype (CD8+) tumor-associated T cells in a biological sample derived from said patient that express the marker Programmed cell Death- 1 (PD-1) and that do not express appreciable amounts of Granzyme B (GzmB); and (b) identifying the subject as a suitable candidate for treatment with said inhibitor by taking into account if a percentage of the number of said CD8+ T cells express PD-1 (PD-1+) and a percentage of the number of the CD8+PD-1+ T cells do not express appreciable amounts of GzmB. Tumor-associated macrophages expressing MHC II and a monocytic marker may also be used in stratification.