NEW PRIMER AND GENOTYPING METHOD OF SLA-1 USING GENOMIC PCR BASED DIRECT SEQUENCING

摘要

The present invention relates to a method for analyzing SLA-1 genotype, and more specifically, to a method for analyzing SLA-1 genotype through direct sequencing of genomic DNA. According to the present invention, genotype of all SLA-1 alleles can be precisely and easily analyzed through PCR and direct sequencing based on genomic DNA without allele information and cloning step, by designing a primer set which is suitable for genomic PCR, amplifies all alleles, and minimizes variability of the allele in amplification efficiency.