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NOVEL PRIMER AND METHOD FOR ANALYZING SLA-2 GENOTYPE BY USING DIRECT SEQUENCING BASED ON GENOMIC PCR
NOVEL PRIMER AND METHOD FOR ANALYZING SLA-2 GENOTYPE BY USING DIRECT SEQUENCING BASED ON GENOMIC PCR
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机译:基于基因组PCR的直接测序的SLA-2基因型的新型底漆和分析方法
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摘要
The present invention relates to a method for analyzing SLA-2 genotype, and more specifically, to a method for analyzing SLA-2 genotype through direct sequencing of genomic DNA. According to the present invention, genotype of all SLA-2 alleles can be precisely and easily analyzed through PCR and direct sequencing based on genomic DNA without allele information and a cloning step, by designing a primer set which is suitable for genomic PCR, amplifies all alleles, and minimizes variability of the allele in amplification efficiency.;COPYRIGHT KIPO 2015
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