Genetically engineered protein and monoclonal antibody of foot-and-mouth disease virus type SAT1 and their application to the diagnostic method

摘要

invention of foot-and-mouth disease virus type SAT1 gene recombinant protein antigens, monoclonal antibodies and using them relates to a diagnosis method. As an example of the type of foot-and-mouth disease diagnosis method using a SAT1 SAT1-type foot-and-mouth disease virus antigen and recombinant protein monoclonal antibody of the present invention (1) can be carried out from step to (11) step. (1) reacting the SAT1-type foot-and-mouth disease virus antibody on the plate; (2) removing by washing the antibody is not attached to the plate; (3) reaction of the foot-and-mouth disease virus type SAT1 gene recombinant protein as a diagnostic antigen in the plate; (4) removing by washing the foot-and-mouth disease virus type SAT1 gene recombinant protein that is not attached to the plate; (5) reacting the test serum sample that is applied to the plate; (6) removing by washing the test serum sample that is not attached to the plate; (7) the step of putting the reaction SAT1-type foot-and-mouth disease virus monoclonal antibody to the plate; 8, foot-and-mouth disease virus type SAT1 removing mAb did not adhere to the plate; (9) reacting a call threshold dish peroxidase enzyme is an anti-mouse antibody coupled to the plate; (10) removing by washing the anti-mouse antibody is attached to the non-call threshold dish plate coupled to a peroxidase enzyme; (11) SAT1 measuring the absorbance of the color reaction by the enzyme reaction by comprising the step of measuring the foot-and-mouth disease virus type SAT1 antibody levels in serum samples testing of foot-and-mouth disease type diagnosis. ;