Avian paramyxovirus type 9 recombinant hemagglutinin-neuraminidase protein expressed by baculovirus and diagnostic method for Avian paramyxovirus type 9 using the same

摘要

the present invention is expressed by chopping Circular virus in birds para myxovirus -9 using this recombinant protein and HN birds para mikso relates to a method for assessing virus -9. And insect cells transfected APMV-9 HN protein by chopping or ocular virus expressing thereto in accordance with the present invention produces APMV-9 HN protein at a high concentration, producing a large amount of antigen in common laboratory cell culture capable of easily using this can and insect cell culture solution to the diagnostic antigen can be produced in a week, so that the liquid diagnostic antigen production time can be shortened, which is effective for at least a week. Further, the APMV-9 HN protein antigens produced by the present invention and the hemagglutination activity and the thermal stability of the chicken red blood cells, APMV-9 is HI reaction by the immune serum, as well as appeared specifically, conventional antigen ( APMV-9 when the virus antigen) response method and HI test results and compared the effect indicating the same test results. Thus, securing an infectious virus, and does not need to APMV-9 can be used to replace the conventional APMV-9 virus antigen as a diagnostic fluid APMV-9 HN protein antigen produced by the present invention. ;