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Development of high-throughput quantitative glycomics platform using mass spectrometry and chromatography system

机译:利用质谱和色谱系统开发高通量定量糖组学平台

摘要

The present invention relates to a super high-speed sugar analyzing method using a mass spectrometer and a chromatography system. The super high-speed sugar analyzing method according to the present invention sequentially extracts, refines, and labels sugar on a 96-well plate, and quantitatively analyzes a particular sugar labeled through an isotope by using MALDI-TOF MS and UPLC, so that sugar analyzing time and costs can be remarkably reduced and sugar in a glucoprotein can be relatively and absolutely quantified.
机译:本发明涉及一种使用质谱仪和色谱系统的超高速糖分析方法。根据本发明的超高速糖分析方法,依次在96孔板上提取,精制和标记糖,并使用MALDI-TOF MS和UPLC对通过同位素标记的特定糖进行定量分析,从而得到糖可以显着减少分析时间和成本,并且可以相对绝对地量化糖蛋白中的糖。

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