Ratiometric two-photon fluorescent probe for detecting hydrogen sulfide and method for preparing the same and quantitative imaging method using the same

摘要

The present invention relates to a kind of changeable colour two-photon fluorescence probe, fluorescence color by with in H_2S human bodies and its manufacturing method. In addition,The present invention relates to one kind for the H_2S that photographs in human body and cell quantification method, by Two Photon Fluorescence, using changeable colour two-photon fluorescence probe, wherein changeable colour two-photon fluorescence probe shows that powerful fluorescent dye passes through simultaneously selectively to colour with H_2S or cytoplasm mitochondria; Cell is easily loaded by small-molecular-weight and excellent dissolubility water; And be whose depth in the tissue for allow to discover H_2S units and human body between 100 and 200 it; M, thus the distribution and activity taken pictures and the mitochondria quantitative approach of cell such as H_2S in human body or cytoplasm. ;The 2015 of copyright KIPO submissions