Ratiometric two-photon fluorescent probe for detecting hydrogen sulfide and method for preparing the same and quantitative imaging method using the same

摘要

the invention in vivo hydrogen sulfide (H ₂ S ) and reaction with two-photon fluorescent probe directed to a variable color and a method for the fluorescent color is changed. The invention also relates to a method for imaging a two-photon microscope to quantitatively with the hydrogen sulfide present in the cells or living tissue, using the variable-color two-photon fluorescent probe. Variable-color two-photon fluorescent probe according to the invention is reacted with mitochondria or selectively stained soon as hydrogen sulfide at the same time in the cytoplasm (H ₂ S) exhibit a strong fluorescent color change, good solubility and a small molecular weight of water as can be loaded because of the ease in the cell, it is possible to detect and optionally hydrogen sulfide from a living tissue cell and the 100 ~ 200 m depth for more than 60 minutes, the cells and the distribution and activity of the hydrogen sulfide within the mitochondria, or cytoplasm from the living body tissue it is possible to quantitatively imaged. ;