A method for measuring in vivo cytotoxicity in earthworm coelomocytes

摘要

The present invention is a kind of for measuring living body earthworm coelomocyte cytotoxicity and combinations thereof, more particularly, to a kind of novel sensitive determination the two kinds of earthworm in vivo cytotoxicity Eiseniaandrei and Peryonyxexcavates of method with and combinations thereof. For this purpose, method includes the following steps: that extracts earthworm coelomocyte is exposed to copper in soil from earthworm, extracted coelomocyte is dyed with calcein-AM (calcein acetyl-o-methyl ester), and identical by flow cytometric analysis. ;The 2015 of copyright KIPO submissions