Method of drug loading in cell-derived membrane vesicles by engineering parent cells with membrane fusogenic liposomes and drug delivery method using thereof

摘要

The present invention relates to a membrane vesicle in which a prepared drug is included using a membrane fusogenic liposome (MFL), a membrane vesicle preparation method, and a drug delivery method using the membrane vesicle. Specifically, the membrane vesicle of the present invention allows hydrophilic and hydrophobic drugs to be simultaneously included therein, is isolated from a cell to facilitate more efficient and selective drug delivery to neighboring cells, and is safe as a nanomaterial derived from a living body, and a step for directly entrapping the drug in the membrane vesicle is unnecessary. Therefore, the membrane vesicle can be effectively used for preventing and treating a variety of cancers and degenerative diseases in which the membrane vesicle is known to play an important role.