HIGHLY EFFICIENT RECOMBINANT ESCHERICHIA COLI STRAINS FOR CO-PRODUCTION OF HYDROGEN AND ETHANOL AND HIGHLY EFFICIENT METHOD FOR CO-PRODUCTION OF HYDROGEN AND ETHANOL FROM GLUCOSE

摘要

The present invention relates to a highly efficient genetically engineered recombinant E. coli strain for co-producing hydrogen and ethanol, and a highly efficient method for co-producing hydrogen and ethanol by using glucose. In the present invention, a related route of E. coli is modified to co-produce highly efficient hydrogen and ethanol; a cell growth becomes possible under anaerobic conditions due to an adaptive evolution of a mutant strain or an enzyme over-expression in a rate-determination step; and the co-production of hydrogen and ethanol becomes possible. The recombinant E. coli according to the present invention has co-produced at a high efficiency hydrogen and ethanol from a fermentation using glucose. A maximum yield of hydrogen and ethanol from glucose is 1.7 and 1.4 mol/mol, respectively. In case of a yield for ethanol, the yield has grown at least by 70% or more compared with wild strains. In addition, an active strain having a PP path, obtained from the present invention, may be utilized in the production and research of a biofuel such as NAD(P)H produced via the PP path, which requires a reducing power, or a useful chemical.