PREPARATION METHOD OF INTRACELLULAR NEURAL STEM CELL FROM INDUCED PLURIPOTENT STEM CELL USING CHIMERA

摘要

The present invention relates to a producing method of neural stem cells in the body from induced pluripotent stem cells (iPSCs) and the method is to prepare chimeric mice using a method which injects iPSCs into blastocyst (or an aggregation method) and to produce neural stem cells by extracting neural stem cells included brain tissues of the chimeric mice from iPSCs. The present invention can be used as a new differentiation technology of iPSCs which cultures neural stem cells in the body by separating neural stem cells from brain tissues of the chimeric mice using iPSCs.