Preparation method of intracellular neural stem cell from induced pluripotent stem cell using chimera

摘要

The present invention relates to a method for producing an inducible pluripotent stem cell-derived neural stem cell using a chimera, which comprises producing a chimeric mouse using a method (or an aggregation method) of injecting pluripotent stem cells into a blastocyst, Derived pluripotent stem cell-derived neural stem cells are extracted to produce neural stem cells. The present invention can be utilized as a new inducible pluripotent stem cell differentiation technique in which neural stem cells are isolated from brain tissue of chimeric mice using induced pluripotent stem cells and cultured in the body of neural stem cells.