POLYMERASE CHAIN REACTION ANALYZING METHOD FOR CONFIRMING NUMBER OF INTRODUCED FOREIGN GENES IN BRASSICACEAE

摘要

The present invention relates to a polymerase chain reaction (PCR) analyzing method for confirming the number of introduced foreign genes in plants from the mutated Brassicaceae genus by an insertion of foreign genes. The present invention comprises: a preparing step for genomic DNA (gDNA) in plants from Brassicaceae genus; an implementing step for the PCR reaction by utilizing a pair of primer for an endogenous genes amplification in the Brassicaceae genus, and a pair of primer specific to the foreign genes; an analyzing step for the number of the introduced foreign genes compared the amplified endogenous genes by the PCR reaction with the amplification amount of the foreign genes. According to the present invention, the number of the introduced foreign genes can be analyzed with a small amount of DNA in a short time introducing the mutated Brassicaceae genus by the insertion of the foreign genes or the other higher plants containing the similar genome structure with Brassicaceae genus.;COPYRIGHT KIPO 2015