A METHOD FOR EVALUATION OF WILD TYPE AND MUTANT TYPE ISOCITRATE DEHYDROGENASE ACTIVITY USING 13C LABELED -KETOGLUTARATE AND NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

摘要

The present invention relates to a method for evaluating the activity of a circular or mutant IDH enzyme by selectively monitoring only α-KG and 2-HG in cell lysates using α-KG labeled with 13 C and a nuclear magnetic resonance spectroscopy technique. The present invention can selectively monitor only α-KG and 2-HG among many compounds in HDF cell lysates using α-KG labeled with 13C, thereby confirming the presence of IDH prototype and mutant type and observing enzyme activity And can be useful for the development of inhibitors of enzymes. In addition, the inhibitor thus developed can be used as a treatment for brain glioma.