-23 -26 METHOD FOR SCREENING -23 AND -26 SIALYLTRANSFERASE VARIANTS AND THEIR APPLICATION FOR SYNTHESIS OF SIALYLOLIGOSACCHARIDES

摘要

present invention sialic acid transferase and mutant variants of how to navigate the characteristics of the It relates to the synthesis of sialic acid oligosaccharides with sialyl transferase gene, and those coding for the variant enzyme. The present invention provides -2,3 sialic acid transfer and improved fasteners Chateau Pasteurella mutant enzyme derived from a protein engineering and photo tumefaciens origin of the -2,6 sialic acid transferase. In addition, sialic acid transferase mutants of the present invention reduces side reactions in the 3 & apos; and 6 & apos; sialyl-oligosaccharide producing reaction, and provides a cost savings from increased productivity and which. ;