BIOCHIP AND METHOD OF TYPING THE GENES OF HEMAGGLUTININ AND NEURAMINIDASE OF THE VIRUS OF INFLUENZA A

摘要

1. A biochip for typing of influenza A virus comprising tipiruyut hybridization probes for the gene of hemagglutinin and neuraminidase covalently attached directly to the glass microarray having a structure discriminating DNA polynucleotides as shown in Table 1 and Table 2, characterized in that the probes each tipiruyut viral subtype immobilized within one mikromassiva.2. A method of typing of influenza A virus, comprising isolating total RNA from a natural sample holding RT-PCR for the amplification of influenza virus genome segments encoding genes neuraminidase and haemagglutinin in the asymmetric mode, using a specific primer oligonucleotides for producing labeled predominantly single-stranded DNA carrying a DNA-DNA hybridizing the amplicons obtained with probes discriminating members of the biochip registration sample labeled hybridization results using the filing present device and interpretation of the results, characterized in that the tags are used as biotin, as a biochip - a microarray as defined in claim 1 as a recording device - a flatbed scanner, and the interpretation of the results is carried out using Scanarray Express program.