METHOD OF CEMENTITIOUS OF THE CAPSULE POLYSACCHARIDE HAEMOPHILUS OF INFLUENZAE TYPE B (HIB) VERSATILE BY ANTIGENS FOR CREATING IMMUNOBIOLOGIC PREPARATIONS

摘要

A method of binding of the capsular polysaccharide of Haemophilus influenzae type b (Hib) antigens carbodiimide method via adipic acid dihydrazide, characterized in that the antigen is a mixture of tetanus and diphtheria toxoids adsorbed on the gel hydroxide or aluminum phosphate, which is centrifuged at 3000 rev / min for 5 minutes, the resulting precipitate bind carbodiimide method via adipic acid dihydrazide with 3 mg capsular polysaccharide of Hib, dissolved in 1 ml of distilled water, 0.9% conjugate washed -n th sodium chloride solution by centrifugation 3000 rpm / min for 5 min and adjusted to 0.9% sodium chloride solution to a volume of 100 ml.