PLASMIDES FOR EXPRESSION OF THE RECOMBINANT BLOOD COAGNITY FACTOR IX OF THE HUMAN, MONOCLONAL LINE OF MAMMAL CELLS - PRODUCER OF THE RECOMBINANT BLOOD AND BLOOD CLEARANCE

摘要

1. A plasmid for expression of a recombinant human coagulation factor IX of a person, in the following sequence essentially containing a region of the beginning of replication of the pUC plasmid, an open reading frame of beta-lactamase (bla) and a prokaryotic promoter of the bla gene; human Epstein-Barr virus terminal terminal repeat segment (EBVTR), functional promoter and first intron of the Chinese hamster alpha 1 elongation factor gene flanked by a 5 'untranslated region of this gene; open reading frame of a gene encoding human coagulation factor IX; encephalomyocarditis virus (EMCV) internal ribosome binding site (IRES); an open reading frame for dehydrofolate reductase (DHFR) expressed as part of a bicistronic mRNA along with a gene encoding human coagulation factor IX; and a functional terminator for the Chinese hamster alpha 1 elongation factor gene, flanked by the 3 'untranslated region of this gene. 2. The plasmid according to claim 1, characterized in that the human blood coagulation factor IX gene encodes a human blood coagulation factor IX polypeptide, the amino acid sequence of which is shown in SEQ ID NO: 2.3. The plasmid according to claim 1, characterized in that the plot of the terminal repeat of the Epstein-Barr virus of a person (EBVTR) is a fragment of the concatemer of the terminal repeat of EBVTR. 4. The plasmid according to claim 1, characterized in that an open reading frame of mouse dehydrofolate reductase (DHFR) is used. The plasmid according to claim 1, characterized in that the plasmid further comprises a Kozak consensus sequence before the sequence encoding the open reading frame of the coagulation factor cr