METHOD OF ESTIMATING PHARMACOLOGICAL AND TOXICOLOGICAL PROPERTIES OF SUBSTANCES - RADIO-, TOXICOPROTECTORS AND RADIO-, TOXICOSENSIBILISERS

摘要

FIELD: medicine.;SUBSTANCE: invention relates to the field of radiobiology and experimental medicine. A method of estimating pharmacological and toxicological properties of substances consist in the following: a substance to be analysed is introduced into a nutritional medium of larvae and flies of Drosophila melanogaster, combining in their genome hypomorphic mutations of ss- and CG5017-genes. The larvae and flies are irradiated with ionising rays with a dose of 1-10 roentgen. Viability, structures of extremities and a level of transcription of CG 1681, CYP6G1 and ss-genes are estimated. The obtained characteristics of the flies, grown on a medium, containing the analysed substance, and the flies, grown on a medium, which does not contain the analysed substance, irradiated and non-irradiated are compared, and pharmacological properties of the substance are determined by the results of comparison of viability, quantity of tarsal segments of extremity and the level of transcription of CG 1681, CYP6G1 and ss-genes in the flies of all formed groups.;EFFECT: method makes it possible to realise effective fast targeted selection and determine the properties of substances with toxicoperotective, radioprotective, toxicosensibilising and radiosensibilising properties.;7 dwg, 2 tbl, 2 ex