METHOD OF PRENATAL AND POSTNATAL DNA-DIAGNOSTICS OF DOWN, EDWARDS PATAU SYNDROME MUTATION delF508 IN GENE OF MUCOVISCIDOSIS AND RHESUS-FACTOR OF FOETUS

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to biotechnology, namely to method of prenatal and postnatal DNA-diagnostics of Down, Edwards and Patau syndrome, mutation delF508 in gene of mucoviscidosis and rhesus-factor of foetus by method of quantitative fluorescent polymerase chain reaction. Method includes isolaion of DNA from analysed material by sorbent Nucleos of the set DIAtom DNA Prep 100. Eluation of DNA by distilled sterile water is carried out. Two multiplex amplifications with application of a set of fluorescent-marked primers are realised - first on 4 loci of the 21^st chromosome, namely D21S1435, D21S1411, D21S11 and IFNAR, on mutation delF508 in gene of mucoviscidosis and on the site of the 10^th exon of gene RhD, second - on 4 loci of the 13^th chromosome, namely D13S628, D13S634, D13S742, D13S305, and 4 loci of the 18^th chromosome, namely D18S386, D18S391, D18S535, D18S978. Obtained PCR-products are mixed in one hole. Detection by method of capillary electrophoresis in polyacrylamide gel is carried out.;EFFECT: invention makes it possible to diagnose presence of syndromes of Patau, Edwards and Down, mutations delF508 in gene of mucoviscidosis and rhesus of foetus belonging with high accuracy.;2 cl, 4 dwg, 1 tbl, 4 ex